Page:Bergey's manual of determinative bacteriology.djvu/137

 Gelatin colonies: Circular, white, convex, smooth, undulate.

Gelatin stab: No liquefaction.

Agar colonies: Circular or amoeboid, white to buff, flat to convex, smooth, entire.

Agar slant: Filiform, pale buff, raised, smooth, undulate.

Broth: Turbid.

Nitrites not produced from nitrates.

Starch not hydrolyzed.

No acid in carbohydrate media.

Attacks phenol and m-cresol.

Aerobic, facultative.

Optimum temperature, between 30° and 35° C.

Source: Isolated from soil.

Habitat: Soil.

43. Pseudonionas stutzeri (Lehmann and Neumann, 1896) Kluyver, 1942. (Bacillus denitrificans II Burri and Stutzer, Cent. f. Bakt., II Abt., 1, 1895, 392; Bacterium stutzeri Lehmann and Neumann, Bakt. Diag., 1 Aufl., 2, 1896, 237; Bacillus nitrogenes Migula, Syst. d. Bakt., 2, 1900, 793; Pseudomonas stutzeri Kluyver, in Koningsberger, Leerb. d. algem. Plantkunde, Scheltema and Holkema, Amsterdam, 2, 1942, 198; not Pseudomonas stutzeri Migula, Syst. d. Bakt., 2, 1900, 929.)

stut′ze.ri. Named for Dr. A. Stutzer, one of the bacteriologists who originally described this species; M.L. gen. noun stutzeri of Stutzer.

Description taken from van Niel and Allen (Jour. Bact., 64, 1952, 421).

Rods, 0.5 to 0.8 by 1.0 to 3.0 microns. Motile, possessing a single polar flagellum. Gram-negative.

Gelatin and agar colonies: Strongly coherent to media, dry consistency later becoming mucoid, resemble craters with elevated ridges which often branch and merge, concentric zones, polygonal elements, granular.

Gelatin: No liquefaction.

Peptone and yeast agar: Good growth.

Broth: Surface film on nitrate- or nitrite-free media which readily breaks up and precipitates.

Potato: Luxuriant, wrinkled, slimy, flesh-to peach-colored growth.

Nitrates, nitrites, nitramines and N2O reduced to elemental nitrogen.

Carbohydrates: No growth when used as a carbon source in mineral media.

Aerobic, facultative.

Optimum pH, 7.0; growth even at pH 9.0.

Optimum temperature, 35° C.

Distinctive characters: Colony shape and consistency, mode and color of potato growth, ability to grow anaerobically in media with nitrate, nitrite, nitramine or N2O, producing foam.

Source: Isolated from soil.

Habitat: Found widely distributed in soil, manure, mud and stagnant water.

44. Pseudomoiias tralucida Kellerman et al., 1913. (Kellerman, McBeth, Scales and Smith, Cent. f. Bakt., II Abt., 39, 1913, 37.)

tra.lu′ci.da. L. adj. tralucidus transparent.

Rods 0.6 by 1.2 microns. Motile with one or two polar flagella. Gram-negative.

Gelatin stab: No liquefaction.

Agar slant: Moderate, flat, glistening, grayish growth.

Broth: Turbid; granular sediment.

Litmus milk: Acid, no coagulation.

Potato: No growth.

Indole not produced.

Nitrites produced from nitrates.

Ammonia not produced.

Starch hydrolysis slight.

Acid from glucose, maltose, lactose, sucrose, starch, glycerol and mannitol.

Attacks cellulose.

Aerobic, facultative.

Optimum temperature, 37° C. Grows also at 20° C.

Habitat: Soil.

45. Pseudomonas lasia Fuller and Norman, 1943. (Jour. Bact., 46, 1943, 275.)

la′si.a. Gr. adj. lasius hairy, rough, shaggy, woolly.

Rods, 0.5 to 0.6 by 1.2 to 2.0 microns, usually occurring singly but sometimes in chains. Motile with a single polar flagellum. Gram-negative.

Gelatin stab: No liquefaction.

Starch agar colonies: Convex, pale yellow, becoming cream color, entire, round.