Page:Bergey's manual of determinative bacteriology.djvu/135

 439; Chester, Man. Determ. Bact., 1901, 308.)

am.bi′gu.a. L. adj. ambiguus going about, hence uncertain.

Small rods, with rounded ends, occurring singly, in pairs and in chains. Motile, possessing a polar flagellum. Gram-negative.

Gelatin colonies: Gray, translucent, slightly raised, irregular, radiate, with transparent margin.

Gelatin stab: No liquefaction.

Agar slant: Gray, limited, entire.

Broth: Turbid, with gray sediment.

Litmus milk: Acid, slowly coagulated. Litmus reduced.

Potato: Gray to creamy, viscid, spreading.

Indole produced.

Aerobic, facultative.

Optimum temperature, between 30° and 35° C.

Source: Isolated from water from the Schuylkill River.

Habitat: Water.

36. Pseudomonas oleovorans Lee and Chandler, 1941. (Jour. Bact., 41, 1941, 378.)

o.le.o′vor.ans. L. oleum oil; L. v. voro to destroy, consume; M.L. part. adj. oleovorans oil-consuming.

Short rods, 0.5 by 0.8 to 1.5 microns, occurring singly and in pairs. Motile. Gram-negative.

Gelatin stab: No liquefaction after 6 weeks.

Gelatin colonies: Up to 1 mm in diameter, fluorescent; similar to agar colonies.

Surface agar colonies: After 24 hours 1 to 2 mm in diameter, smooth, convex, shiny, opaque, creamy, fluorescent by transmitted light. Edge entire in young colonies.

Deep agar colonies: 0.5 by 1.0 to 1.5 mm, lens-shaped, buff-colored, not fluorescent.

Agar slant: Growth raised, smooth, fluorescent, edge erose.

Broth: After 24 hours, moderate turbidity with slight yellowish, viscid sediment. No pellicle or ring. No soluble pigment produced.

Litmus milk: No change.

Indole not produced.

Potato: Good growth.

Nitrites are produced from nitrates.

Starch is hydrolyzed.

No acid from glucose, lactose, sucrose, galactose, xylose, mannitol, salicin or glycerol.

Equally good growth at 25° and 37° C.

Aerobic.

Distinctive character: The fluorescent quality of the colonies is not imparted to any of the artificial media used.

Source: Isolated from cutting compound (oil-water emulsion) circulating in a machine shop. The oil in this compound may be utilized as a sole source of energy.

Habitat: Probably oil-soaked soils. Abundant in cutting compounds.

37. Pseudomonas arvilla Gray and Thornton, 1928. (Cent. f. Bakt., II Abt., 73, 1928, 90.)

ar.vil′.la. L. arvum a field; M.L. dim. noun arvilla a small field.

Rods, 0.5 to 0.7 by 2.0 to 3.0 microns. Motile with one to five polar flagella. Gram-negative.

Gelatin colonies: Circular, whitish, convex, smooth, glistening, lobate.

Gelatin stab: No liquefaction.

Agar colonies: Circular or amoeboid, white to buff, flat to convex, smooth, glistening, opaque, entire.

Agar slant: Filiform, whitish, convex, smooth, ringed, entire.

Broth: Turbid.

Nitrites not produced from nitrates.

Starch not hydrolyzed.

Acid from glucose.

Attacks naphthalene.

Aerobic, facultative.

Grows at 37° C.

Source: Isolated from soil.

Habitat: Soil.

38. Pseudomonas dacunhae Gray and Thornton, 1928. (Cent. f. Bakt., II Abt., 73, 1928, 90.)

da.cun′hae. d'Acunha, place name. Island; M.L. gen. noun dacunhae of d'Acunha.

Rods 0.5 to 0.8 by 1.5 to 3.0 microns. Motile with one to six polar flagella. Gram-negative.

Gelatin colonies: Circular, whitish, raised, smooth, glistening, entire.

Gelatin stab: No liquefaction.