Page:Bergey's manual of determinative bacteriology.djvu/132

 Motile, possessing a polar flagellum. Gram-negative.

Gelatin colonies: Circular, convex, glistening, greenish, translucent. The medium becomes blue-green, fluorescent.

Gelatin stab: Light green, raised, glistening surface growth. No liquefaction. Medium becomes blue-green fluorescent.

Agar slant: Moist, translucent, glistening, light greenish. The medium assumes a greenish color.

Broth: Turbid, becoming greenish.

Litmus milk: No coagulation; alkaline.

Potato: Pale brown, spreading.

Indole not produced.

Aerobic, facultative.

Little or no growth at 35° to 36° C.

Source: From water from the Schuylkill River.

Habitat: Water.

28. Pseudomonas eisenbergii Migula, 1900. (Fluorescirender Bacillus No. 18, Eisenberg, Bakt. Diag., 1 Aufl., 1886, Taf. 7; Bacillus fluorescens non liquefaciens Eisenberg, Bakt. Diag., 3 Aufl., 1891, 145; Migula, Syst. d. Bakt., 2, 1900, 913; Pseudomonas non-liquefaciens Bergey et al.. Manual, 1st ed., 1923, 132.)

eis.en.ber′gi.i. Named for James Eisenberg, the bacteriologist who first described this species; M.L. gen. n. eisenbergii of Eisenberg.

Short, slender rods with rounded ends. Non-motile. Gram-negative.

Gelatin colonies: Fern-like surface colonies. Medium around colonies has a pearly luster.

Gelatin stab: Surface growth has fluorescent shimmer. Scant growth along stab. No liquefaction.

Agar slant: Greenish growth.

Broth: Turbid, fluorescent.

Litmus milk: Unchanged.

Potato: Diffuse, brownish layer. The surface acquires a grayish blue color.

Indole not produced.

Nitrites produced from nitrates.

Acid from glucose.

Blood serum liquefied.

Aerobic.

Optimum temperature, 25° C.

Not pathogenic.

Habitat: Water.

29. Pseudomonas erythra Fuller and Norman, 1943. (Jour. Bact., 46, 1943, 276.)

e′ry.thra. Gr. adj. erythrus red.

Rods, 0.2 to 0.4 by 1.2 to 1.5 microns, with rounded ends, usually arranged singly. Motile with a single polar flagellum. Encapsulated. Gram-negative.

Gelatin stab: No growth.

Starch agar: No growth.

Water-insoluble dextrin agar: Scant growth. Subsurface colonies appear after 8 to 10 days. Colonies are angular, small, surrounded by a clear zone 2 to 5 mm in diameter. Buff or reddish brown.

Litmus milk: No growth.

Indole not produced.

Nitrites not produced from nitrates.

Starch not hydrolyzed.

No growth in media containing the usual carbohydrates. Cellulose and water-insoluble dextrins are utilized. Filter paper strips in mineral solution develop reddish brown spots above the surface of the liquid. Solution becomes cloudy. Colonies enlarge and become viscous, and the paper becomes reddish. The filter paper does not break with moderate shaking but may be wound up in a slimy mass. In cellulose media a reddish, water-soluble pigment is produced.

Yeast extract and nitrate are suitable nitrogen sources.

Aerobic.

Grows in a range from 22° to 35° C.

Source: Isolated from soil.

Habitat: Soil.

30. Pseudomonas fragi (Eichholz, 1902) Huss, 1907, emend. Hussong et al., 1937. (Bacterium fragi Eichholz, Cent. f. Bakt., II Abt., 9, 1902, 425; Huss, Cent. f. Bakt., II Abt., 19, 1907, 661; Hussong, Long and Hammer, Iowa Agr. Exp. Sta. Res. Bull. 225, 1937, 122.)

fra′gi. L. neut. n. fragum strawberry; L. gen. n. fragi of the strawberry.

Description from Hussong, Long and Hammer (loc. cit.).

Rods, 0.5 to 1.0 by 0.75 to 4.0 microns, occurring singly, in pairs and in chains.