Page:Bergey's manual of determinative bacteriology.djvu/131

 Memoirs Nat. Acad. Sci., 7, 1895, 436; Chester, Man. Determ. Bact., 1901, 323.)

in.cog′ni.ta. L. adj. incognitus not examined, unknown.

Short rods, with rounded ends, occurring singly, in pairs and in chains. Motile, possessing a polar flagellum. Gram-negative.

Gelatin colonies: Thin, translucent, slightly granular, becoming greenish. Margin undulate. The medium assumes a blue-green fluorescence.

Gelatin stab: No liquefaction.

Agar slant: Thin, moist, translucent. Agar becomes greenish.

Broth: Turbid, becoming greenish. Pellicle and whitish sediment form.

Litmus milk: Slightly acid in a month. Litmus slowly reduced.

Potato: Moist, glistening, spreading, brown.

Indole is produced (trace).

Aerobic, facultative.

No growth at 35° to 36° C.

Comment: Wright (op. cit., 1895, 441) described an organism that is very similar to this species except that it may produce a faint brownish green coloration in a gelatin stab; Wright named the organism Bacillus nexibilis (Bacterium nexibilis Chester, Ann. Rept. Del. Col. Agr. Exp. Sta., 9, 1897, 74; Pseudomonas nexibilis Chester, op. cit., 1901, 309).

Source: Isolated from water from the Schuylkill River.

Habitat: Water.

25. Pseudomonas rugosa (Wright, 1895) Chester, 1901. (Bacillus rugosus Wright, Memoirs Nat. Acad. Sci., 7, 1895, 438; Chester, Man. Determ. Bact., 1901, 323.)

ru.go′sa. L. adj. rugosus full of wrinkles.

Small rods, with rounded ends, occurring singly, in pairs and in chains. Motile, possessing 1 to 4 polar flagella. Gram-negative.

Gelatin colonies: Grayish, translucent, slightly raised, irregular, sinuous, radiately erose to entire.

Gelatin stab: Dense grayish green, limited, wrinkled, reticulate surface growth. No liquefaction. Medium becomes green.

Agar slant: Grayish white, limited, slightly wrinkled, translucent. Agar becomes green.

Broth: Turbid, with thin whitish pellicle and sediment.

Litmus milk: Acid, coagulated, partly reduced.

Potato: Moist, glistening, brown, spreading.

Indole is produced (trace).

Aerobic.

Optimum temperature, 30° C. Does not grow at 35° C.

Source: From water from the Schuylkill River.

Habitat: Water.

26. Pseudomonas mildenbergii Bergey et al., 1930. (Der Blaubacillus, Mildenberg, Cent. f. Bakt., II Abt., 56, 1922, 309; Bergey et al., Manual, 3rd ed., 1930, 172.)

mil.den.ber′gi.i. Mildenberg, a patronymic; M.L. gen. noun mildenbergii of Mildenberg.

Rods, 0.3 to 0.5 by 1.0 to 3.5 microns, with rounded ends, occurring singly. Motile, possessing polar flagella. Gram-negative.

Gelatin colonies: Circular, lobed, smooth, glistening, slightly raised, steel-blue, entire.

Gelatin stab: No liquefaction.

Agar colonies: Small, circular, yellowish or reddish yellow, entire, becoming lobed, grayish green, iridescent. The medium becomes dirty grayish green.

Agar slant: Smooth, spreading, slimy, glistening, grayish green to dark green, fluorescent.

Broth: Turbid green, iridescent to opalescent with slimy sediment.

Litmus milk: Not coagulated, blue ring.

Potato: Slimy, glistening, spreading, steel blue.

Indole not produced.

Nitrites not produced from nitrates.

Aerobic, facultative.

Optimum temperature, 25° C.

Source: Isolated from air.

27. Pseudomonas convexa Chester, 1901. (Bacillus fluorescens convexus Wright, Memoirs Nat. Acad. Sci., 7, 1895, 438; Chester, Man. Determ. Bact., 1901, 325.)

con.vex′a. L. adj. convexus vaulted, convex.

Short, thick rods, with rounded ends.