Page:Bergey's manual of determinative bacteriology.djvu/128

 Agar slant: Grayish, glistening, translucent, limited. Agar becomes brownish green.

Broth: Turbid, with slight gray pellicle and sediment. Broth becomes green.

Litmus milk: Alkaline; reduction of litmus; slight coagulation. Serum becomes green.

Potato: Thin, brownish, moist, glistening, viscid.

Indole not produced.

Aerobic, facultative.

Optimum temperature, between 20° and 25° C. No growth at 35° C.

Source: From water from the Schuylkill River.

Habitat: Water.

16. Pseudomonas septica Bergey et al., 1930. (Bacillus fluorescens septicus Stutzer and Wsorow, Cent. f. Bakt., II Abt., 71, 1927, 113; Bergey et al., Manual, 3rd ed., 1930, 169.)

sep′ti.ca. Gr. adj. septicus putrefactive, septic.

Rods, 0.6 to 0.8 by 0.8 to 2.0 microns, occurring singly. Motile with a polar flagellum. Gram-negative.

Gelatin stab: Infundibuliform liquefaction.

Agar colonies: Circular with opalescent center and transparent periphery.

Agar slant: Moderate, undulate margin.

Broth: Turbid with fragile pellicle, greenish in upper portion.

Litmus milk: Alkaline, coagulated.

Blood serum not liquefied.

Acid from glucose.

Aerobic, facultative.

Optimum temperature, 20° C.

Source: Isolated from diseased caterpillars.

Habitat: From infected caterpillars so far as known.

17. Pseudomonas syncyanea (Ehrenberg, 1840). Migula, 1895. (Vibrio syncyaneus Ehrenberg, Berichte ü.d. Verh. d. k. Preuss. Akad. d. Wissensch. z. Berlin, 5, 1840, 202; Migula, in Engler and Prantl, Die natürl. Pflanzenfam., 1, 1a, 1895, 29.)

syn.cy.a′ne.a. Gr. syn- along with, entirely; Gr. cyaneus dark blue, dark; M.L. adj. syncyaneus entirely blue.

Rods with rounded ends, occurring singly, occasionally in chains, 0.7 by 2.0 to 4.0 microns. Motile with two to four polar flagella. Gram-negative.

Gelatin colonies: Flat, bluish, translucent.

Gelatin stab: Surface growth shiny, grayish blue. The medium is colored steel-blue with greenish fluorescence. Gelatin is liquefied. Some strains do not liquefy.

Agar slant: Grayish white streak. The medium takes on a bluish gray color with slight fluorescence.

Broth: Turbid with marked fluorescence.

Litmus milk: Unchanged. In association with lactic-acid bacteria the milk takes on a deep blue color.

Potato: Yellowish gray, shiny layer, becoming bluish gray. The tissue becomes bluish gray.

Indole not produced.

Nitrites not produced from nitrates.

Aerobic, facultative.

Optimum temperature, 25° C.

Source: From milk that was bluish in color.

Habitat: The cause of blue milk.

18. Pseudomonas iodinum (Davis, 1939) Tobie, 1939. (Chromobacterium iodinum Davis, Zent. f. Bakt., II Abt., 100, 1939, 273; Tobie, Bull. Assoc. Diplômés Microbiol. Fac, Nancy, No. 18, 1939, 16.)

i.o.di′num. M.L. neut. noun iodinum iodine.

Rods, 0.5 by 1.0 to 2.0 microns, occurring singly. Non-motile. Gram-negative.

Gelatin stab: Stratiform liquefaction. Crystals of iodinin form.

Agar colonies: Round, smooth, gray-white, moist, glistening. Dark purple crystals having the appearance of iodine crystals form in the growth and in the adjacent medium. This pigment is actually a phenazine di-N-oxide, there being no iodine present (Clemo and Mcllwain, Jour. Chem. Soc, Pt. 1, 1938, 479; Clemo and Daglish, Jour. Chem. Soc, Pt. 1, 1950, 1481).

Broth: Turbid. Crystals of iodinin form on bottom of tube.

Litmus milk: Alkaline; slow reduction of litmus.