Page:Bergey's manual of determinative bacteriology.djvu/127

 Indole not produced.

Acid but no gas from glucose, fructose, galactose and glycerol. No acid or gas from salicin or raffinose.

Aerobic.

Grows well at 20° C.

Distinctive character: Produces an intense, diffusible, yellow to orange color in cream or in the cream layer of milk.

Source: Isolated from bitter milk.

Habitat: Milk and cream so far as is known.

13. Pseudoinonas fluorescens Migula, 1895. (Bacillus fluorescens liquefaciens Flügge, Die Mikroorganismen, 1886, 289; Migula, in Engler and Prantl, Die natürl. Pflanzenfamilien, 1, 1a, 1895, 29.)

flu.o.res′cens. L. fluor a flux; M.L. fluoresco to fluoresce; fluor-spar, a fluxing mineral which is fluorescent; M.L. part. adj. fluorescens fluorescing.

Rods, 0.3 to 0.5 by 1.0 to 1.8 microns, occurring singly and in pairs. Motile, possessing a polar flagellum; occasionally non-motile. Gram-negative.

Gelatin colonies: Circular, with greenish center, lobular, liquefying quickly; occasionally viscid.

Gelatin stab: Infundibuliform liquefaction, with whitish to reddish gray sediment.

Agar slant: Abundant, reddish layer, becoming reddish gray. The medium shows greenish to olive-brown coloration.

Broth: Turbid, flocculent, with yellowish green pellicle and grayish sediment.

Litmus milk: No coagulation; becoming alkaline.

Potato: Thick, grayish yellow, spreading, becoming light sepia-brown in color; occasionally viscid.

Indole not produced.

Nitrates reduced to nitrites and ammonia.

Acid from glucose.

Blood serum liquefied.

Aerobic.

Optimum temperature, between 20° and 25° C.

Not pathogenic.

Source: Water, sewage, feces.

Habitat: Soil, water and occasionally foodstuffs that have become contaminated from these sources.

14. Pseudomonas pavonacea Levine and Soppeland, 1926. (Bull. No. 77, Iowa State Agr. College, 1926, 41.)

pa.vo.na′ce.a. L. adj. pavonaceus like a peacock's tail, variegated.

Rods, 0.5 by 4.5 microns, with truncate ends, occurring singly and in chains. Old cells develop 2 to 4 knob-like processes. Sluggishly motile. Gram-negative.

Gelatin stab: Crateriform liquefaction. Medium becoming brown.

Agar colonies: Circular, raised, becoming green, amorphous, entire.

Agar slant: Green, smooth, glistening, viscid, medium becoming dark brown.

Broth: Turbid, with viscid sediment. Medium turned brown.

Litmus milk: Slightly alkaline. Litmus reduced. Peptonized after 10 days.

Potato: No growth.

Hydrogen sulfide produced.

Indole not produced.

Neither nitrites nor gas produced from nitrates.

Blood serum liquefied in 5 days.

No acid or gas from glucose, lactose, sucrose or glycerol.

Aerobic, facultative.

Optimum temperature, 22° C. Scant growth at 37° C.

Distinctive characters: Growth on solid media distinctly green. Not fluorescent. Medium becomes brown.

Source: Isolated from activated sludge.

15. Pseudomonas geniculata (Wright, 1895) Chester, 1901. (Bacillus geniculatus Wright, Memoirs Nat. Acad. Sci., 7, 1895, 459; Chester, Man. Determ. Bact., 1901, 313.)

ge.ni.cu.la'ta. L. adj. geniculatus jointed.

Medium-sized rods, occurring singly, in pairs and in chains. Motile, possessing 1 to 4 polar flagella. Gram-negative.

Gelatin colonies: Circular, whitish, assume a greenish shimmer, translucent. Deep colonies yellowish.

Gelatin stab: Infundibuliform liquefaction. Sediment light pink.