Page:Bergey's manual of determinative bacteriology.djvu/123

 glistening, yellowish gray to creamy. Disagreeable odor. Medium becomes brownish gray.

Indole not produced.

Nitrites not produced from nitrates.

Acetylmethylcarbinol not produced.

Hydrogen sulfide not produced.

Slightly acid, becoming alkaline in glucose. No acid from arabinose, xylose, lactose, sucrose, maltose, trehalose, raffinose, mannitol, dulcitol, inositol or salicin.

Starch not hydrolyzed.

Pathogenic for guinea pigs and rabbits, horned lizards, Gila monsters and chuckwallas. Marked hemolysis of rabbit cells and slight hemolysis of Gila monster cells suspended in agar.

Temperature relations: Optimum, 20° to 25° C. Maximum, 37° C. A retest of several strains of this organism by Haynes shows that it grows well at 37° C. and is closely related to, though not identical with, Pseudomonas aeruginosa Migula.

Distinctive characters: Yellowish green fluorescence present only in meat infusion media. The pigment is water-soluble, but insoluble in chloroform. Pathogenic for guinea pigs, rabbits, horned lizards and chuckwallas.

Source: Isolated from a bacterial disease of horned lizards and Gila monsters.

Habitat: Pathogenic for lizards.

4. Pseudomonas caviae Scherago, 1936. (Jour. Bact., 31, 1936, 83; also see Jour. Inf. Dis., 60, 1937, 245.)

ca′vi.ae. M.L. fem. noun Cavia generic name of the guinea pig; from So. American Indian, "cabiai", a guinea pig; caviae of Cavia.

Rods, 0.6 to 1.0 by 1.5 to 3.0 microns, occurring singly and in pairs; rounded ends. Motile by means of 1 to 3 polar flagella. Encapsulated. Gram-negative.

Gelatin stab: Infundibuliform liquefaction.

Agar colonies: Circular, convex, smooth, iridescent and translucent, finely granular, entire.

Agar slant: Growth abundant, grayish white, butyrous, filiform, glistening, translucent, markedly iridescent. Medium eventually tinged greenish yellow, becoming brownish yellow.

Broth: Cloudy, pellicle, abundant light yellow granular sediment, becoming brown. Medium becomes yellow.

Litmus milk: Acidified, coagulated, peptonized, litmus partially reduced.

Potato: Growth scant, filiform, glistening, light yellow to light orange, becoming light brown.

Hydrogen sulfide not produced.

Indole produced.

Nitrites produced from nitrates.

Blood serum not liquefied.

Blood not hemolyzed.

Sodium formate decomposed.

Catalase-negative.

Methyl red positive; acetylmethylcarbinol not produced.

Citrate broth: No growth.

Methylene blue reduced.

Acid but no gas from glucose, fructose, galactose, maltose, cellobiose, mannitol, lactose, arabinose, sucrose, trehalose, sorbitol, mannose, dextrin, salicin, glycerol, aesculin, amygdalin and starch. No acid from xylose, dulcitol, rhamnose, inulin, adonitol, raffinose, erythritol or inositol.

Aerobic, facultative.

Optimum temperature, 37° C. Grows at 25° C.

Source: Isolated from guinea pigs dead from epizootic septicemia.

Habitat: From infected guinea pigs so far as known.

5. Pseudomonas boreopolis Gray and Thornton, 1928. (Cent. f. Bakt., II Abt., 73, 1928, 92.)

bo.re.o′po.lis. Gr. boreas north; Gr. polis a city; M.L. fem. gen. n. boreopolis of North City.

Rods, 0.5 to 1.0 by 2.0 to 3.0 microns, occurring singly and in pairs. Motile with one to five polar flagella. Gram-negative.

Gelatin colonies: Liquefied.

Gelatin stab: Liquefied. Medium reddened by some strains.

Agar colonies: Circular or amoeboid, white to buff, flat to convex, smooth, glistening, translucent border.

Agar slant: Filiform, whitish, raised, smooth, glistening, fluorescent.